Biosafety Written Program

Link: Biosafety Program

1. Scope of the Biosafety Program
2. Expectations
3. Personnel Responsibilities
4. University Biosafety Committee
5. Records
6. Employee Training
7. Biohazardous Waste
8. Bloodborne Pathogens
9. Aerosol Transmissible Diseases
10. Immune Competence and Susceptibility 
11. Biohazard Use Authorizations

Link: Biological Risk Groups

1. Biosafety Levels of Containment
2. Recombinant or Synthetic Nucleic Acid Molecules (RSNA)
3. Select Agents
4. Animal Subjects
5. Human Subjects

Link: Standard Microbiological Practices

1. Hygiene and HouseKeeping
2. Personal Protective Equipment (PPE)
3. Security and Access 

Link: Biosafety Level 2 Containment Practices 

1. Access
2. Personal Protection & Clothing
3. Signs and Labels
4. Laboratory Space
5. Contamination and Exposure Prevention
6. Decontamination Basics
7. Sharps and Universal Precautions
8. Biohazardous Materials Spills

Link: Biological Waste Section

1. Basic Requirements
2. General Program Rules
3. Labeling Biological Waste
4. Biological Waste Categories
5. Non-hazardous biological Waste (BSL-1)
6. Medical and Biohazardous Waste Storage (BSL-2) 
7. Pathology Waste
8. Sharps Waste
9. Chemical Disinfection
10. Sterilization at SFSU
11. Autoclave Use Policy
12. Requirements for Autoclaving Waste
13. Problems with Autoclaves

Link: Laboratory Biological Waste Webpage

Disinfection with Bleach Protocols

1. To make a 10% bleach solution, you must use household bleach with at least a 5% - 9% sodium hypochlorite concentration. Check the label on the bleach bottle to make sure of the concentration.
2. Always make fresh bleach solution. Old bleach solutions lose effectiveness within 24 hours.
3. Soak for at least 30 minutes to ensure disinfection.
4. Liquid cell cultures and liquid animal blood that are treated by disinfection with bleach, may be disposed of in the sink drain. 

To make a 10% bleach solution, here are some tips:

• Use room temperature water
• Mix 1/3 cup of bleach per gallon of water
• Mix 4 teaspoons of bleach per quart (32 oz) of water
• Mix 2 teaspoons of bleach in a 16 oz spray bottle filled with water

For more information about chemical disinfection protocols, visit the Chemical Disinfection and Disinfection Basics sections of the Biosafety Program Manual.

Appendix A - Review and Update Log

Appendix B - Biological Waste Disposal Chart

Appendix C - Aersol Transmissible Disease Program - Lab

1. Assessing Risk and Controls
2. ATD Exposure Control/Biosafety Plan